P168 INCREASED AGGREGATION OF PROTEOGLYCAN COMPLEXES BY CHONDROITIN SULFATES ADDED TO IL-1 TREATED CHONDROCYTES IS ASSOCIATED WITH DECREASED ADAMTS-5 EXPRESSION

Depletion of aquaporin 1 decreased ADAMTS-4 expression in human chondrocytes

Inflammation serves an important role in the progression of osteoarthritis (OA), and IL‑1β may act as a catabolic factor on cartilage, reducing the synthesis of primary cartilage components type II collagen and aggrecan. Aquaporin 1 (AQP1) is a 28‑kDa water channel formed of six transmembrane domains on the cell membrane. AQP1 is highly expressed in the anus, gallbladder and liver, and is moder...

Enhanced expression of fibronectin by cmd/cmd chondrocytes and its modulation by exogenously added proteoglycan.

Cartilage matrix deficiency (cmd/cmd) in mice is a genetic disorder associated with the failure of chondrocytes to synthesize the core protein of cartilage proteoglycan monomer (cartilage-PG). Immunohistochemical and biochemical analyses revealed enhanced accumulation of fibronectin in culture of cmd/cmd chondrocytes as well as in mutant cartilage tissue in vivo. Purified cartilage-PG, when add...

Expression of ADAMTS-1, ADAMTS-4, ADAMTS-5 and TIMP3 by hepatocellular carcinoma cell lines.

Little is known about the expression or role of ADAMTS-1, -4 and -5 and their endogenous inhibitor TIMP3 in the liver in physiological and pathological conditions. Their expression was, therefore, investigated in the hepatocellular carcinoma cell lines HepG2 and HuH-7 using qRT-PCR and western blotting, and their cellular localisation by immunocytochemistry. Cytokine treatments were used to ass...

Expression in Human Chondrocytes Regulate IL-1-Induced ADAMTS-4 Adaptor Proteins and Ras Synergistically

Increased IL-17A but Decreased IL-27 Serum Levels in Patients with Multiple Sclerosis

Background: Effector CD4+ T cell subsets play an important role in Multiple Sclerosis (MS). Interleukin-27 (IL-27) suppresses Th (Th1, Th2 and Th17) cells and dampens autoimmunity and tissue inflammation by promoting the generation of Type 1 regulatory T cells (Tr1). Objective: To identify the relative levels of IL-27 and IL-17A in MS disease. Method: In a case-control study, venous blood was c...